SELECTION OF POTENTIAL PROBIOTIC LACTOBACILLI FROM PIG FAECES TO BE USED AS ADDITIVES IN PELLETED FEEDING

Abstract

Abstract Thirty-five isolates from pig feces were identified as Lactobacillus reuteri (12 strains), Lactobacillus mucosae (7), Lactobacillus plantarum (6), Lactobacillus kitasatonis (3), Lactobacillus rossiae (2), Lactobacillus ultunensis (2), Lactobacillus crispatus (2), and Lactobacillus intestinalis (1)by partial sequence analysis of the 16S rRNA. All isolates were detected at 8–9 log CFUg −1 . Preliminarily, strains were selected based onresistance to heat treatments (ca. 70 ◦ C for 10 s). The decrease in viability for some L. reuteri , L. mucosae , L. plantarum , L. kitasatonis ,and L. rossiae strains was lower than 1 log cycle. Selected strains were further characterized for acid and bile salt resistance, and antibacterial activity.Except for L. kitasatonis , tolerance to simulated gastric and intestinal conditions was enhanced for all strains by addition of reconstituted skimmedmilk. Antibacterial activity was found against Gram-positive and -negative potential pathogens. L. reuteri