Publication | Closed Access
Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution
117
Citations
7
References
2001
Year
Bioorganic ChemistryError Prone PcrEngineeringMolecular BiologyDna ShufflingEnzyme-catalyzed ReactionChiral EsterBiochemical EngineeringBiochemistryBiocatalysisDirected EvolutionCatalysisComplete ReversalAsymmetric CatalysisEnantioselective SynthesisNatural SciencesEnzyme CatalysisBiotechnologySynthetic Biology
The combination of error prone PCR at high mutation rate and DNA shuffling can be used to invert the direction of enantioselectivity of a lipase-catalyzed hydrolytic kinetic resolution involving a chiral ester.
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