EFFECT OF SEA BUCKTHORN (<i>HIPPOPHAE RHAMNOIDES</i> L.) BERRY EXTRACTS ON THE ACTIVITY OF LIPASE AND LIPOXYGENASE

Abstract

ABSTRACT The inhibitory effect of various extracts (lipophilic, hydrophilic‐EtOH and hydrophilic‐MeOH) from berries of six sea buckthorn cultivars, grown in North‐Eastern Poland and Byelorussia, on lipase and lipoxygenase activities was investigated. The total content of carotenoids in lipophilic and hydrophilic‐MeOH extracts ranged from 293 to 816 mg/100 g and from 5.35 to 6.89 mg/100 g, respectively. β‐Carotene comprised between 84.3 and 95.6% of total carotenoids. The hydrophilic‐MeOH extracts were rich sources of phenolics, but lower quantities of phenolics were also detected in both lipophilic and hydrophilic‐EtOH extracts. The content of vitamin C in hydrophilic‐EtOH extracts was 93–406 mg/100 g. Lipase activity was reduced by 40–70% and 13–50% by lipophilic and hydrophilic‐EtOH extracts, respectively. The effect of hydrophilic‐MeOH extract on lipase activity was negligible. The inhibitory effect of sea buckthorn extracts on lipoxygenase activity was even greater than those exerted on lipase activity.