Photoaffinity Labeling of Small‐Molecule‐Binding Proteins by DNA‐Templated Chemistry

Abstract

DNA-templated affinity labeling: Accurate characterization of small-molecule (SM)–protein interactions is one of the most important but also very challenging tasks in chemical biology and drug discovery. A novel method named DNA-programmed photoaffinity labeling is reported. The introduction of DNA encoding and template effect enable multiplexed protein labeling by multiple probes.