Characterization of Cholylglycine Hydrolase from a Bile-Adapted Strain of <i>Xanthomonas maltophilia</i> and Its Application for Quantitative Hydrolysis of Conjugated Bile Salts

Abstract

Purified bile salt hydrolase from bile-adapted Xanthomonas maltophilia displays Michaelis-Menten kinetics on cholylglycine and cholyltaurine and hydrolyzes bile salts also in crude bovine bile. The protein is a dimer and is resistant to proteinases and to heating at 55 to 60 degrees C for up to 60 min, in agreement with calorimetric data.