High‐Throughput Identification of Substrate Specificity for Protein Kinase by Using an Improved One‐Bead‐One‐Compound Library Approach

Abstract

Pick and choose: The identification of the substrate specificity of a protein kinase is critical in understanding its role and function in a cellular signal transduction network. A high-throughput platform was developed for the identification of tyrosine kinase substrate specificity by using an improved one-bead-one-compound ladder peptide library and MALDI-TOF MS. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z700195_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.