Aerobic Incubation of <i>Clostridium perfringens</i> in the Presence of an Oxygen‐Reducing Membrane Fraction

Abstract

ABSTRACT The recovery and growth rates of C. perfringens were evaluated in media to which an oxygen‐reducing membrane fraction from E. coli was added. Each of three C. perfringens strains was incubated in Tryptose Sulfite Cycloserine agar, with and without the membrane fraction. Recoveries were greater on media incubated aerobically with the membrane fraction than on the other formulations incubated an‐aerobically or aerobically. C. perfringens ATCC 12917 reached stationary phase more rapidly in fluid thioglycollate medium (FTM) plus the membrane fraction than in FTM alone. Results showed that the membrane fraction allowed aerobic incubation for the recovery of C. perfringens .