Preparation of recombinant thioredoxin fused N-terminal proCNP: Analysis of enterokinase cleavage products reveals new enterokinase cleavage sites

A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm

Edward R. LaVallie, Elizabeth Diblasio, Sharlotte Kovacic et al. · Nature Biotechnology · 1993 · 931 citations

Life Sciences, Genetics, Molecular Biology +11

Expression in <i>Escherichia coli</i> of a Chemically Synthesized Gene for the Hormone Somatostatin

Keiichi Itakura, Tadaaki Hirose, Roberto Crea et al. · Science · 1977 · 851 citations

Life Sciences, Biochemistry, Molecular Biology +17

Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid.

P H Hirel, M J Schmitter, P Dessen et al. · Proceedings of the National Academy of Sciences · 1989 · 765 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +11

Strategies for achieving high-level expression of genes in Escherichia coli.

Savvas C. Makrides · Microbiological Reviews · 1996 · 577 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17

Endothelial production of C-type natriuretic peptide and its marked augmentation by transforming growth factor-beta. Possible existence of "vascular natriuretic peptide system".

Shigeru Suga, Kazuwa Nakao, Hiroshi Itoh et al. · Journal of Clinical Investigation · 1992 · 527 citations · Full text

Life Sciences, Neurobiology, Physiology +11