Concepedia

Cryopreservation of Encapsulated Liver Spheroids for a Bioartificial Liver: Reducing Latent Cryoinjury Using an Ice Nucleating Agent

Isobel Massie, Clare Selden, Humphrey Hodgson, Barry Fuller

Tissue Engineering Part C Methods · 2011 · 62 citations · 36 references

DOI

Abstract

Spheroids cryopreserved without INA displayed latent cryoinjury in the first 6 h after thawing. INA reduced supercooling during CryoP and also latent cryoinjury. Cell numbers, viability, and function as measured over 72 h post-thaw were all improved when INA was present during CryoP.

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