Chemical Communications · 2006 · 52 citations · 16 references
Life SciencesBiochemistryMolecular BiologyEnzymologyProtein BiochemistryProteomicsCombinatorial Peptide SynthesisEnzyme ScreeningPeptide AnalysisProtein MicroarraysProteolytic EnzymesProteolytic ProcessingCleavage SpecificityMicroarray AnalysisMicroarray BiosensorsPeptide MicroarraysProtease AssaysProtease Cleavage SpecificityProtease Substrate SpecificityProtease-catalyzed Peptide Cleavage
A 10,000 member PNA-encoded library of FRET based peptides was synthesised for global analysis of protease cleavage specificity; analysis was achieved using a DNA microarray and consumed minimal quantities of enzyme (60 pmole) and library (3.5 nmole).
16
Francis Crick, J. S. Griffith, Leslie E. Orgel · Proceedings of the National Academy of Sciences · 1957 · 329 citations · Full text
Bradley J. Backes, Jennifer L. Harris, Francesco Leonetti et al. · Nature Biotechnology · 2000 · 248 citations