Concepedia

Optimization of cell culture conditions for production of biologically active proteins

Shinji Hosoi, Hiromasa Miyaji, Mitsuo Satoh, Tsukasa Kurimoto, Akira Mihara, Nobuo Fujiyoshi, Seiga Itoh, Seiji Sato

Cytotechnology · 1991 · 16 citations · 45 references

DOI

Concepts

References

45

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

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Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

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A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation

Gray D. Shaw, Robert Kamen · Cell · 1986 · 4.1K citations

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SV40-transformed simian cells support the replication of early SV40 mutants

Yakov Gluzman · Cell · 1981 · 2.5K citations

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A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots

M. S. Blake, Kenneth H. Johnston, G.J. Russell-Jones et al. · Analytical Biochemistry · 1984 · 2.1K citations

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