Rapid Communications in Mass Spectrometry · 1999 · 80 citations · 7 references
PharmacyPharmacokineticsPharmacologyAnalytical ChemistryMass Spectrometric AnalysisMetabolic StabilityBioanalytical Method ValidationHepatic Drug MetabolismIn Vitro Metabolic AssaysMass SpectrometryMetabolite QuantificationMicrosomal StabilityData ProcessingLiquid Chromatography-mass SpectrometryLiquid Chromatography-mass Spectrometry AnalysisMass Spectrometric ScreeningMetabolic Stability ScreeningMetabolic Stability TestingMicrosomal Incubation AssaysPharmacokinetic Parameters
There is a continuing need for increased throughput in the evaluation of new drug entities in terms of their pharmacokinetic parameters. One useful parameter that can be measured in vitro using liver microsomal preparations is metabolic stability. In this report, we describe an automated system that can be used for unattended quantitative analysis of liver microsomal samples for a series of compounds. This system is based on the Sciex API 150 (single quadrupole) liquid chromatography/mass spectrometry system and utilizes 96-well plate autosampler technology as well as a custom-designed AppleScript which executes the on-line data processing and report generation. It has the capability of analyzing at least 75 compounds per week or 300 compounds per month in an automated fashion.
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Timothy Olah, Debra A. McLoughlin, John D. Gilbert · Rapid Communications in Mass Spectrometry · 1997 · 150 citations