Lipase‐catalyzed transesterification of trilinolein or trilinolenin with selected phenolic acids

Abstract

Abstract The enzymatic transesterification of selected phenolic acids with TAG, including trilinolein (TLA) and trillinolenin (TLNA), was investigated in an organic solvent medium. Maximal bioconversion of 66% was obtained with a dihydrocaffeic acid (DHCA) to TLA ratio of 1∶2 after 5 d of reaction. Similarly, the highest bioconversion of 62% was obtained with a DHCA to TLNA ratio of 1∶2, but after 12 d of reaction. However, a ratio of 1∶4 DHCA/TLA decreased the bioconversion to 53%. Transesterification reactions of ferulic acid with both TAG, using a ratio of 1∶2, resulted in low bioconversion of 16 and 14% with TLA and TLNA, respectively. The overall results indicated that bioconversion of phenolic MAG was higher than that of phenolic DAG. The structures of mono‐ and dilinoleyl dihydrocaffeate as well as those of mono‐ and dilinolenyl dihydrocaffeate were confirmed by LC‐MS analyses. The phenolic lipids demonstrated moderate radical‐scavenging activity.