STUDIES ON YEAST DIFFERENTIATION USING ORGANIC ACID METABOLITES PART 1. DEVELOPMENT OF METHODOLOGY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

The separation of organic acids on the column Aminex HPX-87H from Bio-Rad is described. The relationship between column temperature and retention time was studied for both sulphuric and phosphoric acid eluants and an analytical scheme using 0.2% (v/v) phosphoric acid was developed. While the procedure is designed for the commonly occurring non-volatile acid metabolites it was demonstrated that conditions could be established for the analysis of fatty acids to C10. Difficulties in the qualitative and quantitative analysis of organic acids in beer are discussed.