HPLC Determination of Fructose, Glucose, and Sucrose in Potatoes

Abstract

ABSTRACT A high‐performance liquid chromatographic (HPLC) method has been developed to determine fructose, glucose, and sucrose content of potatoes. The HPLC system consisted of a μBondapak/ carbohydrate column, a solvent system of acetonitrile‐water (75: 25), a flowrate of 1.8 ml/min, and a refractive index detector. Analysis, including sample preparation, was complete in 30 min. With the exception of high concentrations of sucrose (8 mg/g or above), the method recovered 93% or more of all sugars. The coefficients of variation for the procedure ranged from 1.39‐13.31%. TLC indicated that there were no interfering compounds eluting with any of the three sugars.