Synthesis of Heterobifunctional Protein Fusions Using Copper‐Free Click Chemistry and the Aldehyde Tag

Abstract

Clicking into place: Cu-free click chemistry is combined with the aldehyde tag protein modification strategy to produce heterobifunctional protein fusions. This method relies on linkers that utilize orthogonal triazole and oxime chemistries (see scheme) to expand on conjugation with the fGly residue and enables site-specific protein conjugation to full-length human antibodies.