Aldehyde Tag Coupled with HIPS Chemistry Enables the Production of ADCs Conjugated Site-Specifically to Different Antibody Regions with Distinct in Vivo Efficacy and PK Outcomes

Penelope M. Drake, Aaron E. Albers, Jeanne Baker, +11

Bioconjugate Chemistry

ImmunologyAdcs Conjugated Site-specificallyConjugation SiteChemical BiologyConventional Lysine Conjugate +20

Hydrazino-Pictet-Spengler Ligation as a Biocompatible Method for the Generation of Stable Protein Conjugates

Paresh Agarwal, Romas Kudirka, Aaron E. Albers, +5

Bioconjugate Chemistry

Bioorganic ChemistryEngineeringProtein AssemblyMolecular BiologyChemical Biology +20

Synthesis of Heterobifunctional Protein Fusions Using Copper‐Free Click Chemistry and the Aldehyde Tag

Jason E. Hudak, Robyn M. Barfield, Gregory W. de Hart, +4

Angewandte Chemie International Edition

Bioorganic ChemistryProtein AssemblyMolecular BiologyClick ChemistryChemistry +14

CRISPR-Cas9 screens identify regulators of antibody–drug conjugate toxicity

C. Kimberly Tsui, Robyn M. Barfield, Curt R. Fischer, +7

Nature Chemical Biology

Antibody–drug Conjugate ToxicityMedicineImmunologyGene EditingGenome Engineering +4

Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion

Patrick G. Holder, Lesley Jones, Penelope M. Drake, +5

Journal of Biological Chemistry

EngineeringAldehyde DehydrogenaseBiochemistryNatural SciencesBiocatalysis +5

Generating Site-Specifically Modified Proteins via a Versatile and Stable Nucleophilic Carbon Ligation

Romas Kudirka, Robyn M. Barfield, Jesse M. McFarland, +8

Chemistry & Biology

EngineeringBiochemistryProtein AssemblyNatural SciencesProtein Biosynthesis +8

The Exomer Coat Complex Transports Fus1p to the Plasma Membrane via a Novel Plasma Membrane Sorting Signal in Yeast

Robyn M. Barfield, J. Christopher Fromme, Randy Schekman

Molecular Biology of the Cell

Molecular BiologyCoated VesiclesTransmembrane CargoCytoskeletonCellular Physiology +15

Maytansine-bearing antibody-drug conjugates induce in vitro hallmarks of immunogenic cell death selectively in antigen-positive target cells

Maxine Bauzon, Penelope M. Drake, Robyn M. Barfield, +3

OncoImmunology

Tandem-Cleavage Linkers Improve the In Vivo Stability and Tolerability of Antibody–Drug Conjugates

Stepan Chuprakov, Ayodele O. Ogunkoya, Robyn M. Barfield, +7

Bioconjugate Chemistry

Site-Specific Tandem Knoevenagel Condensation–Michael Addition To Generate Antibody–Drug Conjugates

Romas Kudirka, Robyn M. Barfield, Jesse M. McFarland, +6

ACS Medicinal Chemistry Letters

Drug TargetBioorganic ChemistryChemical BiologyMedicinal ChemistryAntibody Engineering +15