Determination of the Glucosinolate Content in Commercial Rapeseed Loads with a Pocket Reflectometer

Abstract

Abstract A method is proposed which allows the determination of the glucosinolate content in commercial loads of rapeseed within 10 minutes. The procedure is based on the following steps: Homogenization of 10 g seeds, extraction of the glucosinolates with boiling water, enzymatic hydrolysis of the glucosinolates, removal of interfering substances with activated charcoal paper, detection of the liberated glucose using a glucose test paper, measurement of the colour intensity of the test paper with a pocket reflectometer and calculation of the glucosinolate content on basis of the measured values.