Protein engineering of representative hydrolytic enzymes for remediation of organophosphates

Protein stability promotes evolvability

Jesse D. Bloom, Sy T. Labthavikul, Christopher R. Otey et al. · Proceedings of the National Academy of Sciences · 2006 · 1.3K citations · Full text

Life Sciences, Biochemistry, Evolutionary Biology +17

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

Manfred T. Reetz, José Daniel Carballeira · Nature Protocols · 2007 · 866 citations

Life Sciences, Biotechnology, Molecular Biology +15

Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes

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Life Sciences, Biochemistry, Molecular Biology +12

Expanding the Range of Substrate Acceptance of Enzymes: Combinatorial Active‐Site Saturation Test

Manfred T. Reetz, Marco Bocola, José Daniel Carballeira et al. · Angewandte Chemie International Edition · 2005 · 504 citations

Life Sciences, Biochemistry, Biotechnology +14

Purification and Properties of the Phosphotriesterase from Pseudomonas diminuta

David P. Dumas, Steven R. Caldwell, James R. Wild et al. · Journal of Biological Chemistry · 1989 · 496 citations · Full text

Life Sciences, Biochemistry, Enzyme Mechanism +16