Polycistronic expression of a β-carotene biosynthetic pathway in Saccharomyces cerevisiae coupled to β-ionone production

High-level semi-synthetic production of the potent antimalarial artemisinin

Christopher J. Paddon, Patrick J. Westfall, Douglas J. Pitera et al. · Nature · 2013 · 2K citations · Full text

Medicinal Chemistry, Antiparasitic Agent, Medicine +5

Rapid and reliable protein extraction from yeast

Vitaly V. Kushnirov · Yeast · 2000 · 997 citations

Protein Extraction, Yeast Cells, Protein Purification +17

Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes

Maxim Itkin, Uwe Heinig, Oren Tzfadia et al. · Science · 2013 · 574 citations · Full text

The ‘cleavage’ activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring ‘2A-like’ sequences

Michelle Donnelly, Lorraine E. Hughes, Garry A. Luke et al. · Journal of General Virology · 2001 · 540 citations

Viral Replication, Viral Polymerase Structure, Insect Virus Polyproteins +21

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic <i>Mycoplasma genitalium</i> genome

Daniel G. Gibson, Gwynedd A. Benders, Kevin Axelrod et al. · Proceedings of the National Academy of Sciences · 2008 · 486 citations · Full text

One-step Assembly, Genetics, Yeast Recombination +21