Proceedings of the National Academy of Sciences · 2008 · 496 citations · 18 references
Life SciencesMicrobiologyMolecular BiologyGenome AssemblyRecombinationSynthetic BiologyDe Novo Genome AssemblyDna AssemblyGene Library ConstructionGenome SynthesisMolecular Cloning WorkflowsRecombinant Plasmid ConstructionCloning Of Large Dna FragmentsGenome Assembly From Synthetic DnaHomologous Recombination AssemblyLarge-fragment Dna AssemblyRecombination-mediated AssemblySynthetic Chromosome ConstructionSynthetic Yeast GenomeYeast Recombination
We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces cerevisiae by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.
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A. Hinnen, James Hicks, Gerald R. Fink · Proceedings of the National Academy of Sciences · 1978 · 2.2K citations · Full text
Plasmid construction by homologous recombination in yeast
Hong Ma, Sam Kunes, Peter J. Schatz et al. · Gene · 1987 · 568 citations