Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

Mark A. Bradford · Analytical Biochemistry · 1976 · 225.4K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford · Analytical Biochemistry · 1976 · 211.2K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

Improved Prediction of Signal Peptides: SignalP 3.0

Jannick Dyrløv Bendtsen, Henrik Nielsen, Gunnar von Heijne et al. · Journal of Molecular Biology · 2004 · 6.4K citations

Medicine, Bioinformatics, Molecular Biology +10

Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites

Henrik Nielsen, Jacob Engelbrecht, Søren Brunak et al. · Protein Engineering Design and Selection · 1997 · 5.4K citations · Full text

Life Sciences, Bioinformatics, Molecular Biology +17