Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria

Ulf Brockmeier, Michael Caspers, Roland Freudl, +3

Journal of Molecular Biology

Bacillus SubtilisAll Signal PeptidesBiochemistryNatural SciencesSystematic Screening +6

An outer membrane protein (OmpA) of Escherichia coli K-12 undergoes a conformational change during export.

Roland Freudl, Heinz Schwarz, York‐Dieter Stierhof, +3

Journal of Biological Chemistry

Cell surface exposure of the outer membrane protein OmpA of Escherichia coli K-12

Roland Freudl, Sheila MacIntyre, Maria Degen, +1

Journal of Molecular Biology

Cell Surface ExposureProtein SecretionBiochemistryNatural SciencesMembrane Transport +7

An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform

Peter Rohe, Deepak Venkanna, Britta Kleine, +2

Microbial Cell Factories

The nature of information, required for export and sorting, present within the outer membrane protein OmpA of Escherichia coli K-12.

Roland Freudl, Heinz Schwarz, M Klose, +2

The EMBO Journal

Protein SecretionBiochemistryNatural SciencesVirulence FactorBacteriology +6

The efficient export of NADP‐containing glucose‐fructose oxidoreductase to the periplasm of <i>Zymomonas mobilis</i> depends both on an intact twin‐arginine motif in the signal peptide and on the generation of a structural export signal induced by cofactor binding

Dirk Halbig, Thomas Wiegert, Natascha Blaudeck, +2

European Journal of Biochemistry

Signal Peptide Peptidase- and ClpP-like Proteins of Bacillus subtilis Required for Efficient Translocation and Processing of Secretory Proteins

Albert Bolhuis, Arne Matzen, Hanne‐Leena Hyyryläinen, +7

Journal of Biological Chemistry

Secretion ProcessProtein SecretionBiochemistryNatural SciencesBacteriology +8

The LysE superfamily: topology of the lysine exporter LysE of Corynebacterium glutamicum, a paradyme for a novel superfamily of transmembrane solute translocators.

M Vrljic, Jalaj Garg, A. Bellmann, +9

PubMed

Protein ChemistryLyse FamilyBiosynthesisBiochemistryProtein Folding +11

Isolation and Characterization of Bifunctional Escherichia coli TatA Mutant Proteins That Allow Efficient Tat-dependent Protein Translocation in the Absence of TatB

Natascha Blaudeck, Peter Kreutzenbeck, Matthias Müller, +2

Journal of Biological Chemistry

Improvement of Sec-dependent secretion of a heterologous model protein in Bacillus subtilis by saturation mutagenesis of the N-domain of the AmyE signal peptide

Michael Caspers, Ulf Brockmeier, Christian Degering, +2

Applied Microbiology and Biotechnology

Bacillus SubtilisProtein SecretionSec-dependent SecretionBiochemistryAmye Signal Peptide +4