Concepedia

Library construction and evaluation for site saturation mutagenesis

Bradford Sullivan, Adam Z. Walton, Jon D. Stewart

Enzyme and Microbial Technology · 2013 · 47 citations · 29 references

DOI

Concepts

References

29

Molecular cloning: A laboratory manual

Prescott L. Deininger · Analytical Biochemistry · 1990 · 86.2K citations

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An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol

Huanting Liu, James H. Naismith · BMC Biotechnology · 2008 · 1.3K citations · Full text

, , +13

An efficient one-step site-directed and site-saturation mutagenesis protocol

Lei Zheng · Nucleic Acids Research · 2004 · 1.2K citations · Full text

, , +15

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

Manfred T. Reetz, José Daniel Carballeira · Nature Protocols · 2007 · 866 citations

, , +15

An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule.

D. R. Mills, R. L. Peterson, S. Spiegelman · Proceedings of the National Academy of Sciences · 1967 · 649 citations · Full text

, , +15