Concepedia

Site-saturation Targeting

Site-Saturation Targeting is a methodological framework in molecular biology that enables comprehensive mutagenesis by systematically introducing mutations at every position within a specified target site to assess the functional impact of all possible single alterations.

11

Publications

1.3K

Citations

74

Authors

29

Institutions

Publications per year

2017–2026

2

Parents

Publications

11

BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis

Matthew C. Canver, Elenoe C. Smith, Falak Sher et al. · Nature · 2015 · 910 citations

, , +11

Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system

Vincent W. Keng, Kojiro Yae, Tomoko Hayakawa et al. · Nature Methods · 2005 · 114 citations

, , +12

Library construction and evaluation for site saturation mutagenesis

Bradford Sullivan, Adam Z. Walton, Jon D. Stewart · Enzyme and Microbial Technology · 2013 · 47 citations

, , +13

Gene Site Saturation Mutagenesis: A Comprehensive Mutagenesis Approach

Keith A. Kretz, Toby H. Richardson, Kevin A. Gray et al. · Methods in enzymology on CD-ROM/Methods in enzymology · 2004 · 44 citations

, , +10

Distributions of enzyme residues yielding mutants with improved substrate specificities from two different directed evolution strategies

Janahan Paramesvaran, E. G. Hibbert, Angela J. Russell et al. · Protein Engineering Design and Selection · 2009 · 43 citations · Full text

, , +17

1–5 of 11

Authors

74

  1. YN
    Yukio Nakamura

    RIKEN BioResource Research Center, Japan

    1 publications · 910 citations

    1

    h-index

  2. DE
    Dan E. Robertson

    University of Pennsylvania, United States

    1 publications · 44 citations

    1

    h-index

  3. ZK
    Zongbao K. Zhao

    Dalian Institute of Chemical Physics, China

    1 publications · 21 citations

    1

    h-index

  4. JJ
    Junjie Ji

    Institute of Microbiology, China

    1 publications · 12 citations

    1

    h-index

  5. FZ
    Feng Zhang

    University of Minnesota, United States

    1 publications · 910 citations

    1

    h-index

1–5 of 74

Institutions

29

  1. Oregon Health & Science University

    Portland, United States

    1 h-index

    3 publications

    48 citations

  2. Dana-Farber Cancer Institute

    Boston, United States

    1 h-index

    13 publications

    11.8K citations

  3. McGovern Institute for Brain Research

    Cambridge, United States

    1 h-index

    3 publications

    2.7K citations

  4. Howard Hughes Medical Institute

    Chevy Chase, United States

    1 h-index

    2 publications

    1.8K citations

  5. Institute of Microbiology

    Beijing, China

    1 h-index

    6 publications

    72 citations

  6. Zhejiang University of Technology

    Hangzhou, China

    1 h-index

    5 publications

    90 citations

  7. Shenzhen University

    Shenzhen, China

    1 h-index

    1 publications

    18 citations

  8. Eli Lilly (United States)

    Indianapolis, United States

    1 h-index

    1 publications

    43 citations

1–8 of 29

Venues

  1. Biotechnology Letters

    2 publications · 30 citations

    2

    h-index

  2. Protein Engineering Design and Selection

    PEDS · 1 publications · 43 citations

    1

    h-index

  3. Analytical Biochemistry

    1 publications · 16 citations

    1

    h-index

  4. Nature Methods

    1 publications · 114 citations

    1

    h-index

  5. Insect Biochemistry and Molecular Biology

    1 publications · 30 citations

    1

    h-index

1–5