Profiling Ubiquitin Linkage Specificities of Deubiquitinating Enzymes with Branched Ubiquitin Isopeptide Probes

Abstract

Branching out: Novel branched ubiquitin isopeptide activity-based probes (UIPPs) were engineered to profile ubiquitin (Ub) linkage specificity of deubiquitinating enzymes (DUBs) in cell extracts. Using chemical proteomics, a preference towards K48-linked Ub was shown for USP5, USP7, UCH L-3, and UCH L-5, whereas USP19 and USP38 preferred K63-linked Ub. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.