Molecular Biology of the Cell · 2010 · 445 citations · 5 references
PharmacyBiochemistryMolecular BiologyMolecular BiophysicsMolecular RecognitionProtein BiochemistryBinding AffinityLigand BindingProtein BindingProtein-ligand BindingProtein-ligand RecognitionProtein-nucleic Acid InteractionsAffinity AssaysBinding Affinity MeasurementsBinding AssaysBiomolecular Binding AffinityLigand-binding AssaysNucleic Acid Binding AffinityProtein-ligand Binding Assays
The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail to measure the affinity of the reactants for each other. This essay describes simple methods to get the most out of valuable reagents in binding experiments.
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