Food Biotechnology · 2007 · 11 citations · 23 references
Life SciencesBiotechnologyFood ScienceFood PreservationProtein BiochemistryProtein EngineeringProtein Expression EngineeringProtein SynthesisProteins In FoodsProteolytic EnzymesRecombinant Protein ProductionAntifreeze Protein EffectsAntifreeze Protein ExpressionAntifreeze Protein FunctionBacterial Protein ProductionCryogenic ProcessesExtracellular Enzyme ProductionMicrobial ProteasesPeptide Production
Bacillus subtilis is an ideal host for the production of extracellular heterologous proteins. Antifreeze protein (ice structuring protein, ISP) is a potent additive in cryogenic preservation of food products. In this investigation, the first production of a recombinant ISP was achieved by using a high-efficient artificial promoter and a signal peptide from subtilisin YaB. The functional ISP was expressed extracellularly at a high level by a protease deficient host, B. subtilis WB800. This study provides an efficient tool to produce recombinant food peptide additive at a high level and overcomes the problem of natural resources limitation as well.
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