Binding properties and solubility of single-chain T cell receptors expressed in E. coli

Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction

Yung-Chi Cheng, William H. Prusoff · Biochemical Pharmacology · 1973 · 12.8K citations

Cent Inhibition, Inhibitory Activity, Biochemistry +8

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

James S. Huston, Dennis J. Levinson, M Mudgett-Hunter et al. · Proceedings of the National Academy of Sciences · 1988 · 1.6K citations · Full text

E. Coli, Specific Activity, Molecular Biology +18

Single-Chain Antigen-Binding Proteins

Robert E. Bird, Karl D. Hardman, James W. Jacobson et al. · Science · 1988 · 1.5K citations

Three-Dimensional Structure of an Antigen-Antibody Complex at 2.8 Å Resolution

A.G. Amit, Roy A. Mariuzza, Simon E. V. Phillips et al. · Science · 1986 · 1.2K citations

Serial triggering of many T-cell receptors by a few peptide–MHC complexes

Salvatore Valitutti, Sabina Müller, Marina Cella et al. · Nature · 1995 · 1.1K citations

Signal Transduction, Adaptive Immune System, Many T-cell Receptors +13