Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

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Life Sciences, Genetics, Molecular Biology +17

Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system

F. William Studier · Journal of Molecular Biology · 1991 · 806 citations

Life Sciences, Microbiology, Molecular Biology +13

A Comparison between the Sulfhydryl Reductants Tris(2-carboxyethyl)phosphine and Dithiothreitol for Use in Protein Biochemistry

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Life Sciences, Biochemistry, Enzymology +6

Chaperone Coexpression Plasmids: Differential and Synergistic Roles of DnaK-DnaJ-GrpE and GroEL-GroES in Assisting Folding of an Allergen of Japanese Cedar Pollen, Cryj2, in <i>Escherichia coli</i>

Kazuyo Nishihara, Masaaki Kanemori, Masanari Kitagawa et al. · Applied and Environmental Microbiology · 1998 · 432 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17

Protein <i>trans</i> -splicing by a split intein encoded in a split DnaE gene of <i>Synechocystis</i> sp. PCC6803

Hong Wu, Zhuma Hu, Xiang‐Qin Liu · Proceedings of the National Academy of Sciences · 1998 · 377 citations

Dentistry, Ecology, Molecular Biology +16