An ultrasensitive chemiluminescent ELISA for determination of chloramphenicol in milk, milk powder, honey, eggs and chicken muscle

Abstract

A competitive, direct, chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for chloramphenicol (CAP) residues in milk, milk powder, honey, eggs and chicken muscle has been developed.The method gave a detection limit of 0.7 ng L (1 and a linear range of 2.192.4ng L (1 , with the IC 50 of 13.6 ng L (1 under optimal conditions, dramatically better than any previously reported ELISA method for CAP detection.Spiked at levels of 560 ng L (1 in different food samples, recoveries were in the range of 72.1116.0%,with coefficient of variations of 4.220.2%.In a study of incurred residues, the chicken muscle samples diluted 5-, 10-and 20-fold, results obtained by CL-ELISA correlated well with those obtained by gas chromatography with microcell electron capture detector and traditional ELISA.The developed CL-ELISA method is, therefore, suitable for rapid screening trace CAP residues in food samples.