Over expression and analysis of O-glycosylated recombinant human granulocyte colony stimulating factor in Pichia pastoris using Agilent 2100 Bioanalyzer

O-linked sugar chain of human granulocyte colony-stimulating factor protects it against polymerization and denaturation allowing it to retain its biological activity.

Masayoshi Oh‐eda, Masakazu Hasegawa, Kunihiro Hattori et al. · Journal of Biological Chemistry · 1990 · 177 citations · Full text

Life Sciences, Biochemistry, Immunology +15

Effects of Tween 80 and Sucrose on Acute Short-Term Stability and Long-Term Storage at −20 C of a Recombinant Hemoglobin

Bruce A. Kerwin, Martin Heller, Theodore W. Randolph · Journal of Pharmaceutical Sciences · 1998 · 124 citations

Life Sciences, Biochemistry, Metabolic Stability +9

Structural Characterization of Natural and Recombinant Human Granulocyte Colony-Stimulating Factors1

Naoki Kubota, Tetsuro Orita, Kunihiro Hattori et al. · The Journal of Biochemistry · 1990 · 120 citations

Life Sciences, Biochemistry, Immunology +10

Human granulocyte colony stimulating factor (hG-CSF): cloning, overexpression, purification and characterization

Ana LS Vanz, Gaby Renard, Mário Sérgio Palma et al. · Microbial Cell Factories · 2008 · 68 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +16

Process optimization of constitutive human granulocyte–macrophage colony-stimulating factor (hGM-CSF) expression in Pichia pastoris fed-batch culture

Yogender Pal, Amardeep Khushoo, Krishna Jyoti Mukherjee · Applied Microbiology and Biotechnology · 2005 · 53 citations

Life Sciences, Biotechnology, Microbiology +14