Characterization of Pigments from Different High Speed Countercurrent Chromatography Wine Fractions

TLDR

Let's extract content. Background: sentence: "The biphasic solvent system consisting of tert-butyl methyl ether/n-butanol/acetonitrile/water (2/2/1/5, acidified with 0.1% trifluoroacetic acid) was chosen for its demonstrated efficiency in separating anthocyanins." Mechanism: two sentences: first: "A red wine, made from Cabernet Sauvignon (60%) and Tannat (40%) cultivars, was fractionated by high speed countercurrent chromatography (HSCCC)." second: "The different native and derived anthocyanins were identified on the basis of their UV−visible spectra, their elution time on reversed-phase high-performance liquid chromatography (HPLC), and their mass spectra, before and after thiolysis." Combine into one sentence: "A Cabernet Sauvignon/Tannat red wine was fractionated by HSCCC, and native and derived anthocyanins were identified using UV–visible spectra, reversed‑phase HPLC elution times, and mass spectra before and after thiolysis." Findings: multiple sentences: "The HSCCC method allowed the separation of different families of anthocyanin-derived pigments that were eluted in different fractions according to their structures." "The hydrosoluble fraction was almost devoid of native anthocyanins." "Further characterization (glucose quantification, UV−visible absorbance measurements) indicated that it contained flavanol and anthocyanin copolymers in which parts of the anthocyanin units were in colorless forms." "Pigments in the hydrosoluble fraction showed increased resistance to sulfite bleaching and to the nucleophilic attack of water." Combine into one sentence: "HSCCC separated anthocyanin‑derived pigment families into structure‑based fractions; the hydrosoluble fraction lacked native anthocyanins, contained flavanol‑anthocyanin copolymers with colorless units, and its pigments were more resistant to sulfite bleaching and nucleophilic water attack." Other: "Keywords: Wine; HSCCC; anthocyanins; flavanols; adducts; LC/DAD/ESI-MS; derived pigments" This is a keyword list. According to rule, if Other contains essential meta info like code, data, etc. But here it's just keywords.

Abstract

A red wine, made from Cabernet Sauvignon (60%) and Tannat (40%) cultivars, was fractionated by high speed countercurrent chromatography (HSCCC). The biphasic solvent system consisting of tert-butyl methyl ether/n-butanol/acetonitrile/water (2/2/1/5, acidified with 0.1% trifluoroacetic acid) was chosen for its demonstrated efficiency in separating anthocyanins. The different native and derived anthocyanins were identified on the basis of their UV−visible spectra, their elution time on reversed-phase high-performance liquid chromatography (HPLC), and their mass spectra, before and after thiolysis. The HSCCC method allowed the separation of different families of anthocyanin-derived pigments that were eluted in different fractions according to their structures. The hydrosoluble fraction was almost devoid of native anthocyanins. Further characterization (glucose quantification, UV−visible absorbance measurements) indicated that it contained flavanol and anthocyanin copolymers in which parts of the anthocyanin units were in colorless forms. Pigments in the hydrosoluble fraction showed increased resistance to sulfite bleaching and to the nucleophilic attack of water. Keywords: Wine; HSCCC; anthocyanins; flavanols; adducts; LC/DAD/ESI-MS; derived pigments