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The effect of high hydrostatic pressure on the survival of<i>Saccharomyces cerevisiae</i>in model suspensions and beetroot juice
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2013
Year
High Hydrostatic PressureOsmotic StressModel SuspensionsBioenergeticsPhysiologyBeetroot JuiceBiochemical EngineeringBiotechnologyYeast StrainYeastFood EngineeringMicrobiologyFood ProcessingYeast CellsMedicineBiophysicsHealth Sciences
Abstract The inactivation of Saccharomyces cerevisiae NCFB 3191 using high hydrostatic pressure of 300 MPa at 20°C with a holding time of 0, 1, 5 and 10 min was investigated with model suspensions in phosphate-buffered saline and in beetroot juice. The reduction in S. cerevisiae NCFB 3191 in model suspensions was about 5 log after 10 min of pressurization, irrespective of the initial level of cell concentration in the samples (5.4–8.7 log cfu/mL). The baroprotective effect of beetroot juice on yeast cells during pressurization was observed; the reduction was lower and was only 3.5 log (the inoculum was 5.4 log cfu/mL). No sublethal injury among the surviving cells of the studied yeast strain was found. Keywords: Saccharomyces cerevisiae beetroot juicehigh hydrostatic pressure Acknowledgements The study was financed by the Polish Ministry of Science and Higher Education as grant no. 2011/01/B/NZ9/02537. Notes †This paper was presented at the Lth European High Pressure Research Group (EHPRG 50) Meeting at Thessaloniki, Greece, 16–21 September 2012.
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