Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein

Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria

Ulf Brockmeier, Michael Caspers, Roland Freudl et al. · Journal of Molecular Biology · 2006 · 276 citations

Bacillus Subtilis, All Signal Peptides, Biochemistry +8

The PrsA lipoprotein is essential for protein secretion in <i>Bacillus subtilis</i> and sets a limit for high‐level secretion

Vesa P. Kontinen, Matti Sarvas · Molecular Microbiology · 1993 · 213 citations

Simple Cloning via Direct Transformation of PCR Product (DNA Multimer) to Escherichia coli and Bacillus subtilis

Chun You, Xiaozhou Zhang, Y.‐H. Percival Zhang · Applied and Environmental Microbiology · 2011 · 196 citations · Full text

Engineering, Genetics, Dna Analysis +18

Confirmation of the existence of a third family among peptidyl‐prolyl <i>cis/trans</i> isomerases Amino acid sequence and recombinant production of parvulin

Jens‐U. Rahfeld, Karl Peter Rücknagel, Birte Schelbert et al. · FEBS Letters · 1994 · 193 citations

Optimization of Protease Secretion in <i>Bacillus subtilis</i> and <i>Bacillus licheniformis</i> by Screening of Homologous and Heterologous Signal Peptides

Christian Degering, Thorsten Eggert, Michael Puls et al. · Applied and Environmental Microbiology · 2010 · 178 citations · Full text

Protein Secretion, Biosynthesis, Engineering +15