Biotechnology and Bioengineering · 2013 · 88 citations · 17 references
EngineeringBioengineeringBiotechnologyBiochemical EngineeringMetabolic EngineeringMicrobial MetabolismEnzyme ProductionMetabolic Pathway EngineeringMicrobial Metabolic EngineeringMicrobial Metabolite ProductionPathway OptimizationSynthetic Pathway ConstructionButyrate ProductionEscherichia ColiExogenous EnzymesFed-batch CultivationHeterologous Pathway ConstructionHeterologous Pathway ExpressionOptimization Of Heterologous PathwaysSynthetic Scaffolds
Butyrate pathway was constructed in recombinant Escherichia coli using the genes from Clostridium acetobutylicum and Treponema denticola. However, the pathway constructed from exogenous enzymes did not efficiently convert carbon flux to butyrate. Three steps of the productivity enhancement were attempted in this study. First, pathway engineering to delete metabolic pathways to by-products successfully improved the butyrate production. Second, synthetic scaffold protein that spatially co-localizes enzymes was introduced to improve the efficiency of the heterologous pathway enzymes, resulting in threefold improvement in butyrate production. Finally, further optimizations of inducer concentrations and pH adjustment were tried. The final titer of butyrate was 4.3 and 7.2 g/L under batch and fed-batch cultivation, respectively. This study demonstrated the importance of synthetic scaffold protein as a useful tool for optimization of heterologous butyrate pathway in E. coli.
17
Synthetic protein scaffolds provide modular control over metabolic flux
John E. Dueber, Gabriel C. Wu, G. Reza Malmirchegini et al. · Nature Biotechnology · 2009 · 1.2K citations