Synthetic protein scaffolds provide modular control over metabolic flux

John E. Dueber, Gabriel C. Wu, G. Reza Malmirchegini, +5

Nature Biotechnology

EngineeringBiochemistryProtein AssemblyProtein FoldingNatural Sciences +8

A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly

Michael E. Lee, William C. DeLoache, Bernardo Cervantes, +1

ACS Synthetic Biology

EngineeringGeneticsMolecular BiologySynthetic CircuitBiological Computing +20

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

Jesse G. Zalatan, Michael E. Lee, Ricardo Almeida, +8

Cell

EngineeringSynthetic BiologyGenetic EngineeringGenome EngineeringGene Editing +6

BglBricks: A flexible standard for biological part assembly

J. Christopher Anderson, John E. Dueber, Mariana Leguía, +4

Journal of Biological Engineering

Selection of chromosomal DNA libraries using a multiplex CRISPR system

Owen Ryan, Jeffrey M. Skerker, Matthew J. Maurer, +8

eLife

Gene LibrariesEngineeringMultiplex Crispr SystemGeneticsMultiplex Crispr +13

An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose

William C. DeLoache, Zachary N. Russ, Lauren Narcross, +3

Nature Chemical Biology

Enzyme-coupled Biosensor EnablesEngineeringBiochemistryCellular EnzymologyBiochemical Engineering +5

CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window

Shakked O. Halperin, Connor J. Tou, Eric B. Wong, +3

Nature

Enable DiversificationNatural SciencesGeneticsOligonucleotideGenetic Engineering +9

Use of modular, synthetic scaffolds for improved production of glucaric acid in engineered E. coli

Tae Seok Moon, John E. Dueber, Eric Shiue, +1

Metabolic Engineering

BiosynthesisBiomanufacturingE. ColiEngineeringBiocatalysis +11

Reprogramming Control of an Allosteric Signaling Switch Through Modular Recombination

John E. Dueber, Brian J. Yeh, Kayam Chak, +1

Science

EngineeringReverse GeneticsMolecular BiologySynthetic CircuitCytoskeleton +20

De novo design of bioactive protein switches

Robert A. Langan, Scott E. Boyken, Andrew H. Ng, +12

Nature

Natural SciencesDirected EvolutionMolecular BiologySynthetic BiologySynthetic Circuit +3