Concepedia

Temperature Optimization

Temperature Optimization is a methodological framework within protein expression optimization that systematically determines the most conducive thermal conditions to maximize recombinant protein yield, solubility, and functionality while minimizing aggregation and proteolytic degradation.

200

Publications

8.7K

Citations

843

Authors

330

Institutions

Publications per year

2017–2026

74

Parents

Publications

200

Thermofluor-based high-throughput stability optimization of proteins for structural studies

U.B. Ericsson, B.M. Hallberg, George T. DeTitta et al. · Analytical Biochemistry · 2006 · 843 citations

, , +10

High and Low Annealing Temperatures Increase Both Specificity and Yield in Touchdown and Stepdown PCR

Karl Hecker, Kenneth H. Roux · BioTechniques · 1996 · 413 citations · Full text

, , +12

Rapid cycle DNA amplification: time and temperature optimization.

Carl T. Wittwer, D. J. H. Garling · PubMed · 1991 · 241 citations

, , +13

Use of subambient thermal analysis to optimize protein lyophilization

Byeong S. Chang, Cynthia S. Randall · Cryobiology · 1992 · 208 citations

, , +14

A Facile, Water-Soluble Method for Modification of Proteins with DOTA. Use of Elevated Temperature and Optimized pH To Achieve High Specific Activity and High Chelate Stability in Radiolabeled Immunoconjugates

Michael R. Lewis, Andrew Raubitschek, John E. Shively · Bioconjugate Chemistry · 1994 · 163 citations

, , +16

1–5 of 200

Authors

843

  1. DY
    Da‐Chuan Yin

    Northwestern Polytechnical University, China

    5 publications · 103 citations

    5

    h-index

  2. QL
    Qin‐Qin Lu

    Northwestern Polytechnical University, China

    5 publications · 103 citations

    5

    h-index

  3. YG
    Yun‐Zhu Guo

    Northwestern Polytechnical University, China

    4 publications · 79 citations

    4

    h-index

  4. CZ
    Chen‐Yan Zhang

    Northwestern Polytechnical University, China

    4 publications · 90 citations

    4

    h-index

  5. HS
    Hiroki Saeki

    Hokkaido University, Japan

    4 publications · 50 citations

    4

    h-index

1–5 of 843

Institutions

330

  1. Northwestern Polytechnical University

    Xi'an, China

    5 h-index

    39 publications

    840 citations

  2. Eindhoven University of Technology

    Eindhoven, The Netherlands

    4 h-index

    12 publications

    687 citations

  3. Ghent University

    Ghent, Belgium

    4 h-index

    18 publications

    596 citations

  4. Hokkaido University

    Sapporo, Japan

    4 h-index

    4 publications

    50 citations

  5. Central South University

    Changsha, China

    4 h-index

    12 publications

    320 citations

  6. Centre National de la Recherche Scientifique

    Paris, France

    4 h-index

    16 publications

    789 citations

  7. Bristol-Myers Squibb (United States)

    New York, United States

    3 h-index

    15 publications

    595 citations

  8. Doshisha Women's College of Liberal Arts

    Kyoto, Japan

    3 h-index

    4 publications

    53 citations

1–8 of 330

Venues

  1. Biotechnology and Bioengineering

    5 publications · 191 citations

    5

    h-index

  2. Journal of Biotechnology

    4 publications · 58 citations

    4

    h-index

  3. 4

    h-index

  4. Japanese Journal of Applied Physics

    JJAP · 3 publications · 36 citations

    3

    h-index

  5. Physics in Medicine and Biology

    3 publications · 160 citations

    3

    h-index

1–5