Single-molecule Biophysics

Single-molecule biophysics is a subdiscipline of biophysics that investigates the structure, dynamics, and function of individual biological molecules through direct experimental measurement and force manipulation, thereby revealing molecular heterogeneity and behaviors inaccessible through ensemble-averaging methods.

4.7K

Publications

375.1K

Citations

13.7K

Authors

2.3K

Institutions

Publications per year

2017–2026

1.1K

Parents

Biophysics

Children

Atomic Force MicroscopyBiomolecular InteractionsEnsemble MeasurementsLigand Binding KineticsMagnetic TweezersMolecular HeterogeneityMolecular MotorsNucleic Acid DynamicsOptical TweezersPhotophysics Of Fluorescent Probes

Publications

4.7K

RNA velocity of single cells

Gioele La Manno, Ruslan Soldatov, Amit Zeisel et al. · Nature · 2018 · 4.5K citations · Full text

Life Sciences, Cell Biology, Molecular Biology +13

Enhancement and Quenching of Single-Molecule Fluorescence

Pascal Anger, Palash Bharadwaj, Lukáš Novotný · Physical Review Letters · 2006 · 3.1K citations

Natural Sciences, Chemistry, Physics +16

One size fits all?

Franz Tödtling, Michaela Trippl · Research Policy · 2005 · 2.5K citations

Education, Marketing Management, Single-molecule Biophysics +1

Robust single-particle tracking in live-cell time-lapse sequences

Khuloud Jaqaman, Dinah Loerke, Marcel Mettlen et al. · Nature Methods · 2008 · 2.1K citations · Full text

Natural Sciences, Biophysics, Cell Biology +14

Single myosin molecule mechanics: piconewton forces and nanometre steps

Jeffrey T. Finer, Robert Simmons, James A. Spudich · Nature · 1994 · 1.9K citations

Natural Sciences, Biophysics, Physics +16

1–5 of 4.7K

Authors

13.7K

TH
Taekjip Ha
Howard Hughes Medical Institute, United States
58 publications · 12.5K citations
39
h-index
HE
Hermann E. Gaub
Ludwig-Maximilians-Universität München, Germany
40 publications · 7K citations
33
h-index
WE
W. E. Moerner
Stanford University, United States
44 publications · 6K citations
32
h-index
MR
Matthias Rief
Technical University of Munich, Germany
35 publications · 6.4K citations
30
h-index
CB
Carlos Bustamante
University of California, Berkeley, United States
33 publications · 8.2K citations
28
h-index

1–5 of 13.7K

Institutions

2.3K

1–8 of 2.3K

Venues

Proceedings of the National Academy of Sciences
PNAS · 261 publications · 41.6K citations
119
h-index
Biophysical Journal
343 publications · 31.5K citations
91
h-index
Physical Review Letters
PRL · 141 publications · 21.8K citations
74
h-index
Journal of the American Chemical Society
JACS · 162 publications · 13.3K citations
66
h-index
Nature Communications
115 publications · 8K citations
58
h-index

1–5

See also Single-molecule Biophysics
Core Methods	Force Spectroscopy Total Internal Reflection Fluorescence Microscopy Optical Tweezers Bayesian Inference Single-particle Tracking Single-molecule Colocalization Magnetic Tweezers Confocal Microscopy
Biological Applications	Dna Replication Molecular Motors Intracellular Transport Aptamers
Instrumentation	Force Spectroscopy Cryo-electron Microscopy Fluorescence Correlation Spectroscopy Photon-counting Detectors Optical Tweezers
Data Analysis	Bayesian Inference Model Selection Machine Learning Deep Learning Maximum Likelihood Estimation Nonlinear Least Squares Hidden Markov Models Histogram Analysis Supervised Learning Viterbi Algorithm Nonparametric Statistics Information Theory Change-point Detection
Limitations	Photobleaching Ergodicity Ill-posed Inverse Problems Overfitting