Concepedia

Single-guide Rna Design

Single-Guide RNA Design is a computational and experimental framework that specifies the systematic construction of a chimeric RNA molecule, combining the functions of CRISPR RNA (crRNA) and trans-activating crRNA (tracrRNA), to enable precise target recognition and cleavage by Cas9 endonuclease in genome editing applications.

241

Publications

42.6K

Citations

1.6K

Authors

475

Institutions

Publications per year

2017–2026

175

Variants

Sgrna Design

Parents

Publications

241

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench, Nicolò Fusi, Meagan E. Sullender et al. · Nature Biotechnology · 2016 · 5K citations

, , +13

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Bernd Zetsche, Jonathan S. Gootenberg, Omar O. Abudayyeh et al. · Cell · 2015 · 4.9K citations · Full text

, , +12

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

F. Ann Ran, Patrick D. Hsu, Chie-Yu Lin et al. · Cell · 2013 · 3.4K citations · Full text

, , +16

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

Xingliang Ma, Qunyu Zhang, Qinlong Zhu et al. · Molecular Plant · 2015 · 2.4K citations · Full text

, , +16

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

John G. Doench, Ella Hartenian, Daniel B. Graham et al. · Nature Biotechnology · 2014 · 1.7K citations · Full text

, , +17

1–5 of 241

Authors

1.6K

  1. FZ
    Feng Zhang

    Broad Institute, United States

    5 publications · 9.3K citations

    5

    h-index

  2. WL
    Wei Li

    Dana-Farber Cancer Institute, United States

    5 publications · 970 citations

    5

    h-index

  3. HX
    Han Xu

    Dana-Farber Cancer Institute, United States

    4 publications · 819 citations

    4

    h-index

  4. HW
    Hans‐Hermann Wessels

    New York Genome Center, United States

    4 publications · 254 citations

    4

    h-index

  5. HZ
    Houxiang Zhu

    Washington University in St. Louis, United States

    4 publications · 175 citations

    4

    h-index

1–5 of 1.6K

Institutions

475

  1. Harvard University

    Cambridge, United States

    18 h-index

    64 publications

    45.7K citations

  2. Broad Institute

    Cambridge, United States

    13 h-index

    72 publications

    131.8K citations

  3. Howard Hughes Medical Institute

    Chevy Chase, United States

    13 h-index

    42 publications

    27.6K citations

  4. Chinese Academy of Sciences

    Beijing, China

    13 h-index

    79 publications

    16.6K citations

  5. Massachusetts Institute of Technology

    Cambridge, United States

    10 h-index

    48 publications

    74.5K citations

  6. University of Chinese Academy of Sciences

    Beijing, China

    9 h-index

    36 publications

    3.9K citations

  7. University of California, San Francisco

    San Francisco, United States

    8 h-index

    51 publications

    15.9K citations

  8. Seoul National University

    Seoul, South Korea

    6 h-index

    13 publications

    4.5K citations

1–8 of 475

Venues

  1. Genome biology

    10 publications · 2.3K citations

    10

    h-index

  2. Nucleic Acids Research

    9 publications · 2.9K citations

    9

    h-index

  3. Nature Communications

    9 publications · 1.7K citations

    9

    h-index

  4. Bioinformatics

    9 publications · 495 citations

    9

    h-index

  5. Nature Biotechnology

    8 publications · 8.6K citations

    8

    h-index

1–5