Concepedia

Periplasmic Expression Optimization

Periplasmic Expression Optimization is a methodological framework within protein expression optimization that specifically enhances the yield, solubility, and correct folding of recombinant proteins by targeting their expression to the periplasmic space of Gram-negative bacteria and systematically modulating host strains, signal peptides, cultivation conditions, and secretion pathways to improve extracytoplasmic protein localization and recovery.

32

Publications

1K

Citations

145

Authors

58

Institutions

Publications per year

2017–2026

10

Parents

Publications

32

Selection for a periplasmic factor improving phage display and functional periplasmic expression

Hendrick Bothmann, Andreas Plückthun · Nature Biotechnology · 1998 · 198 citations

, , +12

Optimizing Recombinant Protein Production in the Escherichia coli Periplasm Alleviates Stress

Thomas Baumgarten, A. Jimmy Ytterberg, Roman A. Zubarev et al. · Applied and Environmental Microbiology · 2018 · 60 citations · Full text

, , +16

Improvement of the electron transfer rate in Shewanella oneidensis MR-1 using a tailored periplasmic protein composition

Verónica Delgado, Catarina M. Paquete, Gunnar Sturm et al. · Bioelectrochemistry · 2019 · 60 citations · Full text

, , +11

Cosecretion of Chaperones and Low-Molecular-Size Medium Additives Increases the Yield of Recombinant Disulfide-Bridged Proteins

Jörg Schäffner, Jeannette Winter, Rainer Rudolph et al. · Applied and Environmental Microbiology · 2001 · 59 citations

, , +17

New Approaches for Functional Expression of Recombinant Horseradish Peroxidase C In<i>Escherichia Coli</i>

T. A. Chubar, Yury Kapeliuch, Torsten Börchers et al. · Biocatalysis and Biotransformation · 1999 · 48 citations

, , +17

1–5 of 32

Authors

145

  1. JP
    Julián Pérez‐Pérez

    Hospital Universitario Fundación Jiménez Díaz, Spain

    2 publications · 56 citations

    2

    h-index

  2. JL
    José Luís Barbero

    Universidad Autónoma de Madrid, Spain

    2 publications · 56 citations

    2

    h-index

  3. LC
    Leigh C. Bowering

    UCB Pharma (United Kingdom), United Kingdom

    2 publications · 56 citations

    2

    h-index

  4. TD
    Thorben Dammeyer

    Technische Universität Braunschweig, Germany

    1 publications · 28 citations

    1

    h-index

  5. GÅ
    Geir Åge Løset

    University of Oslo, Norway

    1 publications · 24 citations

    1

    h-index

1–5 of 145

Institutions

58

  1. University of Tehran

    Tehran, Iran

    2 h-index

    4 publications

    75 citations

  2. Islamic Azad University, Science and Research Branch

    Tehran, Iran

    1 h-index

    1 publications

    12 citations

  3. Jiangnan University

    Wuxi, China

    1 h-index

    6 publications

    114 citations

  4. Helmholtz Centre for Infection Research

    Braunschweig, Germany

    1 h-index

    1 publications

    28 citations

  5. KTH Royal Institute of Technology

    Stockholm, Sweden

    1 h-index

    3 publications

    129 citations

  6. National Research Council

    Rome, Italy

    1 h-index

    1 publications

    35 citations

  7. Leibniz Association

    Berlin, Germany

    1 h-index

    3 publications

    63 citations

  8. Australian Regenerative Medicine Institute

    Clayton, Australia

    1 h-index

    2 publications

    38 citations

1–8 of 58

Venues

  1. Protein Expression and Purification

    5 publications · 75 citations

    5

    h-index

  2. Applied Microbiology and Biotechnology

    4 publications · 100 citations

    4

    h-index

  3. Journal of Biotechnology

    2 publications · 53 citations

    2

    h-index

  4. Applied and Environmental Microbiology

    AEM · 2 publications · 119 citations

    2

    h-index

  5. Molecular Biotechnology

    1 publications · 32 citations

    1

    h-index

1–5