Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

Ayal Hendel, Rasmus O. Bak, Joseph T. Clark, +12

Nature Biotechnology

Natural SciencesGenetic EngineeringMolecular BiologyHuman Primary CellsGene Editing +7

CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells

Daniel P. Dever, Rasmus O. Bak, Andreas Reinisch, +13

Nature

Crispr/cas9 β-Globin GeneStem Cell ResearchGene EditingGenome EditingMedicine +2

Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

Sriram Vaidyanathan, Krist Azizian, A.K.M. Ashiqul Haque, +8

Molecular Therapy — Nucleic Acids

PCNA Ubiquitination Is Important, But Not Essential for Translesion DNA Synthesis in Mammalian Cells

Ayal Hendel, Peter H.L. Krijger, Noam Diamant, +12

PLoS Genetics

Quantifying Genome-Editing Outcomes at Endogenous Loci with SMRT Sequencing

Ayal Hendel, Eric Kildebeck, Eli J. Fine, +5

Cell Reports

Off-target EffectFunctional GenomicsNatural SciencesGeneticsNext-generation Sequencing +13

Global Transcriptional Response to CRISPR/Cas9-AAV6-Based Genome Editing in CD34+ Hematopoietic Stem and Progenitor Cells

M. Kyle Cromer, Sriram Vaidyanathan, Daniel E. Ryan, +12

Molecular Therapy

DNA damage bypass operates in the S and G2 phases of the cell cycle and exhibits differential mutagenicity

Noam Diamant, Ayal Hendel, Ilan Vered, +5

Nucleic Acids Research

Dna DamageGeneticsMolecular BiologyTranslesion Dna SynthesisCell Cycle +17

CRISPR-based gene editing enables <i>FOXP3</i> gene repair in IPEX patient cells

Marianne Goodwin, E. Lee, Uma Lakshmanan, +19

Science Advances

Different hotspot p53 mutants exert distinct phenotypes and predict outcome of colorectal cancer patients

Ori Hassin, Nishanth Belugali Nataraj, Michal Shreberk‐Shaked, +17

Nature Communications

EngineeringPathologyCancer BiologyTumor BiologyTranscriptional Regulation +16

Nuclease-mediated gene editing by homologous recombination of the human globin locus

Richard A. Voit, Ayal Hendel, Shondra M. Pruett‐Miller, +1

Nucleic Acids Research

GeneticsMolecular BiologyMolecular GeneticsTranscriptional RegulationProtein Expression +19