Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

Mami Matano, Shoichi Date, Mariko Shimokawa, +6

Nature Medicine

Colorectal CancerGenome EngineeringGene EditingGenome EditingSystems Biology +4

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

Masayuki Fujii, Mariko Shimokawa, Shoichi Date, +11

Cell stem cell

Tumor BiologyOncologyTumor HeterogeneityNiche Factor RequirementsMedicine +7

Visualization and targeting of LGR5+ human colon cancer stem cells

Mariko Shimokawa, Yuki Ohta, Shingo Nishikori, +7

Nature

Tumor BiologyBiological MicroenvironmentsCancer GenomicsStem Cell ResearchRadiation Oncology +4

Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression

Takashi Seino, Shintaro Kawasaki, Mariko Shimokawa, +16

Cell stem cell

Tumor BiologyLineage PlasticityPancreatic CancerDisease ProgressionMedicine +7

Human Intestinal Organoids Maintain Self-Renewal Capacity and Cellular Diversity in Niche-Inspired Culture Condition

Masayuki Fujii, Mami Matano, Kohta Toshimitsu, +5

Cell stem cell

BiologyGut-organ AxisMorphogenesisNiche-inspired Culture ConditionDigestive Tract +6

Efficient genetic engineering of human intestinal organoids using electroporation

Masayuki Fujii, Mami Matano, Kosaku Nanki, +1

Nature Protocols

EngineeringBiotechnologyGenetic EngineeringSynthetic BiologyEfficient Genetic Engineering +2

Divergent Routes toward Wnt and R-spondin Niche Independency during Human Gastric Carcinogenesis

Kosaku Nanki, Kohta Toshimitsu, Ai Takano, +20

Cell

Human Gastric CarcinogenesisGastrointestinal OncologyOncogenic AgentMedicineDivergent Routes +6

Somatic inflammatory gene mutations in human ulcerative colitis epithelium

Kosaku Nanki, Masayuki Fujii, Mariko Shimokawa, +18

Nature

InflammationAutoimmune DiseasePathogenesisGastroenterologyPathology +2

An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping

Kenta Kawasaki, Kohta Toshimitsu, Mami Matano, +33

Cell

Neuro-oncologyDevelopmental BiologyNeuroendocrine DisorderCerebral OrganoidNeuroendocrine Tumors +3

Reconstruction of the Human Colon Epithelium In Vivo

Shinya Sugimoto, Yuki Ohta, Masayuki Fujii, +9

Cell stem cell

Human Colon EpitheliumHistopathologyGastroenterologyReconstructive SurgeryMedicine +2