Anti-TMV activity of flavonol derivatives containing piperidine sulfonamide: Design, synthesis, mechanism study

Abstract

A series of flavonol derivatives containing piperidine sulfonamide were designed and synthesized. The EC50 values for the curative of tobacco mosaic virus (TMV) by Z6 and Z22 were 108.2 and 102.7 μg/mL, respectively, outperforming the control agent ningenmycin (NNM) with an EC50 value of 253.8 μg/mL. Additionally, the EC50 values for the protective activity of Z6 and Z21 were 100.9 and 123.2 μg/mL, respectively, also surpassing the control agent NNM with an EC50 value of 203.7 μg/mL. Microscale thermophoresis (MST) and molecular docking test showed that Z6 bound well with tobacco mosaic virus coat protein (TMV-CP). The biological safety test showed that Z6 had almost no effect on seed germination and seedling growth. After treatment with Z6, the chlorophyll content and superoxide dismutase (SOD) activity in tobacco leaves increased, while the malondialdehyde (MDA) content decreased. Therefore, Z6 has a high probability as a potential antiviral agent.