Counteraction of stability-activity trade-off of Nattokinase through flexible region shifting

Enzymatic assembly of DNA molecules up to several hundred kilobases

Daniel G. Gibson, Lei Young, Ray-Yuan Chuang et al. · Nature Methods · 2009 · 10.6K citations

Dna Nanotechnology, Dna Sequencing, Dna +9

Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach

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Analysis of Protein Stability and Ligand Interactions by Thermal Shift Assay

Kathy Huynh, Carrie L. Partch · Current Protocols in Protein Science · 2015 · 569 citations · Full text

Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy

Yinglu Cui, Yanchun Chen, Xinyue Liu et al. · ACS Catalysis · 2021 · 538 citations

Engineering, Bioplastic, Molecular Biology +21

FireProt: web server for automated design of thermostable proteins

Miloš Musil, Jan Štourač, Jaroslav Bendl et al. · Nucleic Acids Research · 2017 · 205 citations · Full text

Protein Folding, Natural Sciences, Directed Evolution +13