Rapid detection of cordycepin in food by surface-enhanced Raman technique

Abstract

In this paper, a method for rapid detection of cordycepin in food was established based on surface-enhanced Raman spectroscopic analysis method. The surface-enhanced Raman substrates were screened and the Raman detection conditions and sample pretreatment methods were optimized. The optimal substrate was gold nanocolloid, and the optimal detection conditions were as follows: the addition amount of gold nanocolloid was 200 μL of gold nanocolloid and the sample addition amount was 5 μL. Under the optimal conditions, the detection limit of cordycepin was 1 mg/L, and the sample pretreatment was performed by methanol extraction. Based on the established surface-enhanced Raman spectroscopy (SERS) method, cordycepin in two Cordyceps militaris was detected, which confirmed the practical application of this method.