Telomere-to-telomere genome assembly of bitter melon (<i>Momordica charantia</i> L. var. <i>abbreviata</i> Ser.) reveals fruit development, composition and ripening genetic characteristics

Abstract

<i>Momordica charantia</i> L. var. <i>abbreviata</i> Ser. (Mca), known as bitter gourd or bitter melon, is a <i>Momordica</i> variety with medicinal value and belongs to the Cucurbitaceae family. In view of the lack of genomic information on bitter gourd and other <i>Momordica</i> species and to promote Mca genomic research, we assembled a 295.6-Mb telomere-to-telomere (T2T) high-quality Mca genome with six gap-free chromosomes after Hi-C correction. This genome is anchored to 11 chromosomes, which is consistent with the karyotype information, and comprises 98 contigs (N50 of 25.4 Mb) and 95 scaffolds (N50 of 25.4 Mb). The Mca genome harbors 19 895 protein-coding genes, of which 45.59% constitute predicted repeat sequences. Synteny analysis revealed variations involved in fruit quality during the divergence of bitter gourd. In addition, assay for transposase-accessible chromatin by high-throughput sequencing and metabolic analysis showed that momordicosides and other substances are characteristic of Mca fruit pulp. A combined transcriptomic and metabolomic analysis revealed the mechanisms of pigment accumulation and cucurbitacin biosynthesis in Mca fruit peels, providing fundamental molecular information for further research on Mca fruit ripening. This report provides a new genetic resource for <i>Momordica</i> genomic studies and contributes additional insights into Cucurbitaceae phylogeny.