Revista do Instituto de Medicina Tropical de São Paulo · 2022 · 41 citations · 9 references
Life SciencesBiologyInfectious DiseasesMetagenomicsMolecular VirologyVirologyDna SequencingShotgun Metagenomic SequencingShotgun MetagenomicsViral InfectionsViral MetagenomicsZoonotic VirusesDna ExtractionMetagenomic SequencingMetagenomic Sequencing Of VirusesSequence GenerationShotgun Metagenomic AnalysisShotgun SequencingZoonotic Viral DiseasesZoonotic Viral Infections
Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.
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The Sequence Alignment/Map format and SAMtools
Heng Li, Alec Wysoker, Tim Fennell et al. · Bioinformatics · 2009 · 67.5K citations · Full text
Minimap2: pairwise alignment for nucleotide sequences
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Using Tablet for visual exploration of second-generation sequencing data
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