Angewandte Chemie International Edition · 2006 · 22 citations · 0 references
EngineeringGeneticsMolecular BiologyIterative CastingMolecular GeneticsEnzymatic ModificationProtein‐sequence SpaceGene StructureIterative CyclesStructure-function Enzyme KineticsLess BranchBiochemistryPage 1237Directed EvolutionGene EvolutionBiologyNatural SciencesEnzyme CatalysisComputational BiologyBiotechnologySynthetic BiologyEnzyme SpecificityGenetic MechanismProtein EvolutionProtein Engineering
The penultimate sentence of the first paragraph on page 1237, just above Figure 1, should read: “In each case, only three libraries are necessary, for example, the gene of the best mutant arising from A is used as a template for randomization at sites B, C, and D, etc. (Figure 1).” In a variation of this iterative CASTing, one less branch occurs in each generation until all sites A, B, C, and D have been utilized with termination of the confined protein sequence space.