Accelerated Procedure for Salmonella Detection in Dried Foods and Feeds Involving Only Broth Cultures and Serological Reactions1

Abstract

A procedure has been developed in which Salmonella can be detected in dried foods and feeds within 50 hr. This includes preenrichment (18 hr), selective enrichment (24 hr), elective enrichment (6 to 8 hr), and serological testing (2 hr). The procedure is as sensitive as the more time-consuming, traditional (plating, biochemical, and serological) procedure which may require at least 4 to 5 days. The new procedure is rapid and accurate in comparison to traditional procedures. Moreover, it is simple and inexpensive to perform. As described, the procedure does not necessitate the isolation of pure cultures, but this step is performed easily if desired.